Cloning and functional analysis of succinate dehydrogenase gene PsSDHA in Phytophthora sojae.
Identifieur interne : 000A48 ( Main/Exploration ); précédent : 000A47; suivant : 000A49Cloning and functional analysis of succinate dehydrogenase gene PsSDHA in Phytophthora sojae.
Auteurs : Yuemin Pan [République populaire de Chine] ; Tao Ye [République populaire de Chine] ; Zhimou Gao [République populaire de Chine]Source :
- Microbial pathogenesis [ 1096-1208 ] ; 2017.
Descripteurs français
- KwdFr :
- Analyse de séquence (MeSH), Chlorure de sodium (métabolisme), Clonage moléculaire (MeSH), Extinction de l'expression des gènes (physiologie), Gènes fongiques (MeSH), Hyphae (croissance et développement), Hyphae (cytologie), Interférence par ARN (MeSH), Maladies des plantes (microbiologie), Milieux de culture (composition chimique), Mutation (MeSH), Peroxyde d'hydrogène (métabolisme), Phytophthora (croissance et développement), Phytophthora (enzymologie), Phytophthora (génétique), Phytophthora (pathogénicité), Phénotype (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Soja (microbiologie), Stress physiologique (MeSH), Succinate Dehydrogenase (génétique), Succinate Dehydrogenase (physiologie), Séquence nucléotidique (MeSH), Virulence (MeSH).
- MESH :
- composition chimique : Milieux de culture.
- croissance et développement : Hyphae, Phytophthora.
- cytologie : Hyphae.
- enzymologie : Phytophthora.
- génétique : Phytophthora, Succinate Dehydrogenase.
- microbiologie : Maladies des plantes, Soja.
- métabolisme : Chlorure de sodium, Peroxyde d'hydrogène.
- pathogénicité : Phytophthora.
- physiologie : Extinction de l'expression des gènes, Succinate Dehydrogenase.
- Analyse de séquence, Clonage moléculaire, Gènes fongiques, Interférence par ARN, Mutation, Phénotype, Régulation de l'expression des gènes fongiques, Stress physiologique, Séquence nucléotidique, Virulence.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Cloning, Molecular (MeSH), Culture Media (chemistry), Gene Expression Regulation, Fungal (MeSH), Gene Silencing (physiology), Genes, Fungal (MeSH), Hydrogen Peroxide (metabolism), Hyphae (cytology), Hyphae (growth & development), Mutation (MeSH), Phenotype (MeSH), Phytophthora (enzymology), Phytophthora (genetics), Phytophthora (growth & development), Phytophthora (pathogenicity), Plant Diseases (microbiology), RNA Interference (MeSH), Sequence Analysis (MeSH), Sodium Chloride (metabolism), Soybeans (microbiology), Stress, Physiological (MeSH), Succinate Dehydrogenase (genetics), Succinate Dehydrogenase (physiology), Virulence (MeSH).
- MESH :
- chemical , chemistry : Culture Media.
- chemical , genetics : Succinate Dehydrogenase.
- chemical , metabolism : Hydrogen Peroxide, Sodium Chloride.
- cytology : Hyphae.
- enzymology : Phytophthora.
- genetics : Phytophthora.
- growth & development : Hyphae, Phytophthora.
- microbiology : Plant Diseases, Soybeans.
- pathogenicity : Phytophthora.
- physiology : Gene Silencing, Succinate Dehydrogenase.
- Base Sequence, Cloning, Molecular, Gene Expression Regulation, Fungal, Genes, Fungal, Mutation, Phenotype, RNA Interference, Sequence Analysis, Stress, Physiological, Virulence.
Abstract
Succinate dehydrogenase (SDH) is one of the key enzymes of the tricarboxylic acid cycle (TCA cycle) and a proven target of fungicides for true fungi. To explore the roles of the SDHA gene in Phytophthora sojae, we first cloned PsSDHA to construct the PsSDHA silenced expression vector pHAM34-PsSDHA, and then utilized PEG to mediate the P. sojae protoplast transformation experiment. Through transformation screening, we obtained the silenced mutants A1 and A3, which have significant suppressive effect. Further study showed that the hyphae of the silenced mutant strains were shorter and more bifurcated; the growth of the silenced mutants was clearly inhibited in 10% V8 agar medium containing sodium chloride (NaCl), hydrogen peroxide (H2O2) or Congo Red, respectively. The pathogenicity of the silenced mutants was significantly reduced compared with the wild-type strain and the mock. The results could help us better to understand the position and function of SDH in P. sojae and provide a proven target of fungicides for the oomycete.
DOI: 10.1016/j.micpath.2017.03.012
PubMed: 28438637
Affiliations:
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Electronic address: gaozhimou@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036</wicri:regionArea><placeName><settlement type="city">Hefei</settlement><region type="province">Anhui</region></placeName></affiliation></author></analytic><series><title level="j">Microbial pathogenesis</title><idno type="eISSN">1096-1208</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Culture Media (chemistry)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Gene Silencing (physiology)</term><term>Genes, Fungal (MeSH)</term><term>Hydrogen Peroxide (metabolism)</term><term>Hyphae (cytology)</term><term>Hyphae (growth & development)</term><term>Mutation (MeSH)</term><term>Phenotype (MeSH)</term><term>Phytophthora (enzymology)</term><term>Phytophthora (genetics)</term><term>Phytophthora (growth & development)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>RNA Interference (MeSH)</term><term>Sequence Analysis (MeSH)</term><term>Sodium Chloride (metabolism)</term><term>Soybeans (microbiology)</term><term>Stress, Physiological (MeSH)</term><term>Succinate Dehydrogenase (genetics)</term><term>Succinate Dehydrogenase (physiology)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence (MeSH)</term><term>Chlorure de sodium (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Extinction de l'expression des gènes (physiologie)</term><term>Gènes fongiques (MeSH)</term><term>Hyphae (croissance et développement)</term><term>Hyphae (cytologie)</term><term>Interférence par ARN (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Milieux de culture (composition chimique)</term><term>Mutation (MeSH)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (enzymologie)</term><term>Phytophthora (génétique)</term><term>Phytophthora (pathogénicité)</term><term>Phénotype (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Soja (microbiologie)</term><term>Stress physiologique (MeSH)</term><term>Succinate Dehydrogenase (génétique)</term><term>Succinate Dehydrogenase (physiologie)</term><term>Séquence nucléotidique (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Succinate Dehydrogenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydrogen Peroxide</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Hyphae</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Hyphae</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hyphae</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Hyphae</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term><term>Succinate Dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chlorure de sodium</term><term>Peroxyde d'hydrogène</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Succinate Dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Silencing</term><term>Succinate Dehydrogenase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Fungal</term><term>Genes, Fungal</term><term>Mutation</term><term>Phenotype</term><term>RNA Interference</term><term>Sequence Analysis</term><term>Stress, Physiological</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence</term><term>Clonage moléculaire</term><term>Gènes fongiques</term><term>Interférence par ARN</term><term>Mutation</term><term>Phénotype</term><term>Régulation de l'expression des gènes fongiques</term><term>Stress physiologique</term><term>Séquence nucléotidique</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Succinate dehydrogenase (SDH) is one of the key enzymes of the tricarboxylic acid cycle (TCA cycle) and a proven target of fungicides for true fungi. To explore the roles of the SDHA gene in Phytophthora sojae, we first cloned PsSDHA to construct the PsSDHA silenced expression vector pHAM34-PsSDHA, and then utilized PEG to mediate the P. sojae protoplast transformation experiment. Through transformation screening, we obtained the silenced mutants A1 and A3, which have significant suppressive effect. Further study showed that the hyphae of the silenced mutant strains were shorter and more bifurcated; the growth of the silenced mutants was clearly inhibited in 10% V8 agar medium containing sodium chloride (NaCl), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) or Congo Red, respectively. The pathogenicity of the silenced mutants was significantly reduced compared with the wild-type strain and the mock. The results could help us better to understand the position and function of SDH in P. sojae and provide a proven target of fungicides for the oomycete.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28438637</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>08</Day></DateCompleted><DateRevised><Year>2018</Year><Month>01</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-1208</ISSN><JournalIssue CitedMedium="Internet"><Volume>108</Volume><PubDate><Year>2017</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Microbial pathogenesis</Title><ISOAbbreviation>Microb Pathog</ISOAbbreviation></Journal><ArticleTitle>Cloning and functional analysis of succinate dehydrogenase gene PsSDHA in Phytophthora sojae.</ArticleTitle><Pagination><MedlinePgn>40-48</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0882-4010(16)30731-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micpath.2017.03.012</ELocationID><Abstract><AbstractText>Succinate dehydrogenase (SDH) is one of the key enzymes of the tricarboxylic acid cycle (TCA cycle) and a proven target of fungicides for true fungi. To explore the roles of the SDHA gene in Phytophthora sojae, we first cloned PsSDHA to construct the PsSDHA silenced expression vector pHAM34-PsSDHA, and then utilized PEG to mediate the P. sojae protoplast transformation experiment. Through transformation screening, we obtained the silenced mutants A1 and A3, which have significant suppressive effect. Further study showed that the hyphae of the silenced mutant strains were shorter and more bifurcated; the growth of the silenced mutants was clearly inhibited in 10% V8 agar medium containing sodium chloride (NaCl), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) or Congo Red, respectively. The pathogenicity of the silenced mutants was significantly reduced compared with the wild-type strain and the mock. The results could help us better to understand the position and function of SDH in P. sojae and provide a proven target of fungicides for the oomycete.</AbstractText><CopyrightInformation>Copyright © 2017. Published by Elsevier Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Yuemin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Zhimou</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China. Electronic address: gaozhimou@126.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microb Pathog</MedlineTA><NlmUniqueID>8606191</NlmUniqueID><ISSNLinking>0882-4010</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>451W47IQ8X</RegistryNumber><NameOfSubstance UI="D012965">Sodium Chloride</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.3.99.1</RegistryNumber><NameOfSubstance UI="D013385">Succinate Dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017421" MajorTopicYN="N">Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012965" MajorTopicYN="N">Sodium Chloride</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013385" MajorTopicYN="N">Succinate Dehydrogenase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phenotypic analysis</Keyword><Keyword MajorTopicYN="N">Phytophthora sojae</Keyword><Keyword MajorTopicYN="N">RNAi</Keyword><Keyword MajorTopicYN="N">Succinate dehydrogenase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>11</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28438637</ArticleId><ArticleId IdType="pii">S0882-4010(16)30731-8</ArticleId><ArticleId IdType="doi">10.1016/j.micpath.2017.03.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Anhui</li></region><settlement><li>Hefei</li></settlement></list><tree><country name="République populaire de Chine"><region name="Anhui"><name sortKey="Pan, Yuemin" sort="Pan, Yuemin" uniqKey="Pan Y" first="Yuemin" last="Pan">Yuemin Pan</name></region><name sortKey="Gao, Zhimou" sort="Gao, Zhimou" uniqKey="Gao Z" first="Zhimou" last="Gao">Zhimou Gao</name><name sortKey="Ye, Tao" 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